Gel-forming Mucins in Oviductus Ranae Contribute to Swelling Capacity by iTRAQ Proteomics Analysis
By: Zhang, He
.
Contributor(s): Zhang, Yuyao.
Publisher: Bangalore Association of Pharmaceutical Teachers of India (APTI) 2021Edition: Vol.55(3), Jul-Sep.Description: 638-648p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Introduction: Oviductus Ranae (OR) is the dried oviduct of female Rana temporaria
chensinensis David, which is one of the best-known and highly valued oriental foods
and medicines in China. OR has a unique physiological phenomenon that is high swelling
capacity. Objectives: We aimed to study which components are associated with high
swelling capacity in OR. Materials and Methods: isobaric tags for relative and absolute
quantification (iTRAQ) proteomic methods were used to identify differentially expressed
proteins between the OR and three counterfeit oviducts, including Rana nigromaculata,
Bufo bufogargarizans and Rana catesbeiana to explore the mechanism of swelling
capacity. Results: We found that a total of 1220 proteins were identified from 2149
unique peptide sequences. Comparing with three counterfeit oviducts, 11 differentially
expressed proteins were identified in OR, including 9 up-regulated proteins, such as
mucins (MUC2, MUC5AC, MUC5AC-like, MUC5B), PREDICTED: a-kinase anchor protein
13-like (AKAP13-like), PREDICTED: IgGFc-binding protein (FcγBP), PREDICTED: IgGFc-
binding protein-like (FcγBP-like), et al. and 2 down-regulated proteins. Bioinformatics
results found that these up-regulated proteins were involved in biological processes,
including metabolic process, cellular process, responses to stimulus and biological
regulation. Conclusion: Mucins, AKAP13-like, FcγBP and FcγBP-like may act as a
positive regulator for the innate immune response in oviduct of Chinese brown frog
and exceptional swelling capacity of OR is closely related to gel-forming mucins.
Taken together, our results provide the basis for further understanding of the unique
physiological phenomenon of OR.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022319 |
Introduction: Oviductus Ranae (OR) is the dried oviduct of female Rana temporaria
chensinensis David, which is one of the best-known and highly valued oriental foods
and medicines in China. OR has a unique physiological phenomenon that is high swelling
capacity. Objectives: We aimed to study which components are associated with high
swelling capacity in OR. Materials and Methods: isobaric tags for relative and absolute
quantification (iTRAQ) proteomic methods were used to identify differentially expressed
proteins between the OR and three counterfeit oviducts, including Rana nigromaculata,
Bufo bufogargarizans and Rana catesbeiana to explore the mechanism of swelling
capacity. Results: We found that a total of 1220 proteins were identified from 2149
unique peptide sequences. Comparing with three counterfeit oviducts, 11 differentially
expressed proteins were identified in OR, including 9 up-regulated proteins, such as
mucins (MUC2, MUC5AC, MUC5AC-like, MUC5B), PREDICTED: a-kinase anchor protein
13-like (AKAP13-like), PREDICTED: IgGFc-binding protein (FcγBP), PREDICTED: IgGFc-
binding protein-like (FcγBP-like), et al. and 2 down-regulated proteins. Bioinformatics
results found that these up-regulated proteins were involved in biological processes,
including metabolic process, cellular process, responses to stimulus and biological
regulation. Conclusion: Mucins, AKAP13-like, FcγBP and FcγBP-like may act as a
positive regulator for the innate immune response in oviduct of Chinese brown frog
and exceptional swelling capacity of OR is closely related to gel-forming mucins.
Taken together, our results provide the basis for further understanding of the unique
physiological phenomenon of OR.
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